Meaning
Phase retrieval techniques resolve the phase problem by collecting intensity measurements under varying optical conditions. The strategy of defocus diversity involves acquiring multiple images of the same object at different defocus positions. These images provide the necessary constraints to reconstruct both the amplitude and the phase of the complex wavefront.
This method avoids the need for complex phase-shifting hardware or interferometers.
Wavefront Sensing
Astronomical imaging and microscopy utilize diverse intensity planes to determine wavefront aberrations. By analyzing how the intensity distribution evolves as the beam propagates through focus, the defocus diversity algorithm calculates the phase of the field. This calculation relies on the transport of intensity equation or iterative algorithms.
The sensitivity of the phase measurement depends on the choice of defocus steps.
Information Redundancy
Acquiring images at multiple focal planes provides a redundant dataset that reduces sensitivity to measurement noise. Since different spatial frequencies are modulated differently at each defocus position, the combination of planes ensures that there are no blind spots in the reconstructed spectrum. This redundancy improves the accuracy of the recovered phase.
However, collecting too many images increases the exposure time and exposes the sample to potential damage or drift.
Computational Cost
Iterative reconstruction from multiple defocused images requires substantial numerical calculation. The algorithm must repeatedly propagate the estimated wavefront back and forth between the measurement planes, updating the amplitude at each step with the observed data. This process demands high performance processors and optimized Fourier transform libraries to achieve reasonable processing times.
If the defocus steps are not calibrated, the algorithm may fail to converge, resulting in a distorted phase map. Therefore, automated stage control and precise calibration of the defocus increments are critical to the success of the reconstruction workflow in high-throughput environments.